expression plasmid prc cmv stat1 y701f Search Results


expression plasmid prc cmv stat1 y701f  (Addgene inc)
90
Addgene inc expression plasmid prc cmv stat1 y701f
FIGURE 4. p38 MAPK inhibition affects H5N1-induced IFN expression. A, impact of p38 MAPK inhibition on the IFN promoter activity. Vero cells were transfected with the IFN promoter for 24 h. Cells were preincubated with 20 M SB 202190 or left untreated and subsequently stimulated with 500 ng of total RNA isolated from infected A549 cells (8 h, 5 m.o.i.). Total RNA from uninfected A549 cells was used as control. 5 h p.s. promoter activity was measured by a luciferase assay and the results are depicted as mean n-fold ( S.D.) of three independent experiments normalized to controls. B, Western blot analysis of total lysates of HUVEC treated with UV-inactivated, filtered conditioned media from mock-infected control cells (lanes 5 and 7) and KAN-1-infected cells (5 m.o.i., 5 h) (lanes 6 and 8). Donor cells were pretreated with DMSO (lanes 1 and 3) or SB 202190 (20 M, lanes 2 and 4). <t>STAT1</t> Tyr701 phosphorylation was detected 15 min aftertreatmentwithconditionedmedium(upperpanel).EqualloadingwasverifiedbythedetectionoftotalERK2(lowerpanel).Blotsarerepresentativeofthree independent experiments.
Expression Plasmid Prc Cmv Stat1 Y701f, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+plasmid+prc+cmv+stat1+y701f/10__1074_slash_jbc__m113__469239-81-1-9?v=Addgene+inc
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expression plasmid prc cmv stat1 y701f - by Bioz Stars, 2026-07
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flag tagged y701f stat1 construct  (Addgene inc)
90
Addgene inc flag tagged y701f stat1 construct
FIGURE 4. p38 MAPK inhibition affects H5N1-induced IFN expression. A, impact of p38 MAPK inhibition on the IFN promoter activity. Vero cells were transfected with the IFN promoter for 24 h. Cells were preincubated with 20 M SB 202190 or left untreated and subsequently stimulated with 500 ng of total RNA isolated from infected A549 cells (8 h, 5 m.o.i.). Total RNA from uninfected A549 cells was used as control. 5 h p.s. promoter activity was measured by a luciferase assay and the results are depicted as mean n-fold ( S.D.) of three independent experiments normalized to controls. B, Western blot analysis of total lysates of HUVEC treated with UV-inactivated, filtered conditioned media from mock-infected control cells (lanes 5 and 7) and KAN-1-infected cells (5 m.o.i., 5 h) (lanes 6 and 8). Donor cells were pretreated with DMSO (lanes 1 and 3) or SB 202190 (20 M, lanes 2 and 4). <t>STAT1</t> Tyr701 phosphorylation was detected 15 min aftertreatmentwithconditionedmedium(upperpanel).EqualloadingwasverifiedbythedetectionoftotalERK2(lowerpanel).Blotsarerepresentativeofthree independent experiments.
Flag Tagged Y701f Stat1 Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+plasmid+prc+cmv+stat1+y701f/10__1091_slash_mbc__e10___02___0112-90-0-13?v=Addgene+inc
Average 90 stars, based on 1 article reviews
flag tagged y701f stat1 construct - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

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FIGURE 4. p38 MAPK inhibition affects H5N1-induced IFN expression. A, impact of p38 MAPK inhibition on the IFN promoter activity. Vero cells were transfected with the IFN promoter for 24 h. Cells were preincubated with 20 M SB 202190 or left untreated and subsequently stimulated with 500 ng of total RNA isolated from infected A549 cells (8 h, 5 m.o.i.). Total RNA from uninfected A549 cells was used as control. 5 h p.s. promoter activity was measured by a luciferase assay and the results are depicted as mean n-fold ( S.D.) of three independent experiments normalized to controls. B, Western blot analysis of total lysates of HUVEC treated with UV-inactivated, filtered conditioned media from mock-infected control cells (lanes 5 and 7) and KAN-1-infected cells (5 m.o.i., 5 h) (lanes 6 and 8). Donor cells were pretreated with DMSO (lanes 1 and 3) or SB 202190 (20 M, lanes 2 and 4). STAT1 Tyr701 phosphorylation was detected 15 min aftertreatmentwithconditionedmedium(upperpanel).EqualloadingwasverifiedbythedetectionoftotalERK2(lowerpanel).Blotsarerepresentativeofthree independent experiments.

Journal: Journal of Biological Chemistry

Article Title: Inhibition of p38 Mitogen-activated Protein Kinase Impairs Influenza Virus-induced Primary and Secondary Host Gene Responses and Protects Mice from Lethal H5N1 Infection

doi: 10.1074/jbc.m113.469239

Figure Lengend Snippet: FIGURE 4. p38 MAPK inhibition affects H5N1-induced IFN expression. A, impact of p38 MAPK inhibition on the IFN promoter activity. Vero cells were transfected with the IFN promoter for 24 h. Cells were preincubated with 20 M SB 202190 or left untreated and subsequently stimulated with 500 ng of total RNA isolated from infected A549 cells (8 h, 5 m.o.i.). Total RNA from uninfected A549 cells was used as control. 5 h p.s. promoter activity was measured by a luciferase assay and the results are depicted as mean n-fold ( S.D.) of three independent experiments normalized to controls. B, Western blot analysis of total lysates of HUVEC treated with UV-inactivated, filtered conditioned media from mock-infected control cells (lanes 5 and 7) and KAN-1-infected cells (5 m.o.i., 5 h) (lanes 6 and 8). Donor cells were pretreated with DMSO (lanes 1 and 3) or SB 202190 (20 M, lanes 2 and 4). STAT1 Tyr701 phosphorylation was detected 15 min aftertreatmentwithconditionedmedium(upperpanel).EqualloadingwasverifiedbythedetectionoftotalERK2(lowerpanel).Blotsarerepresentativeofthree independent experiments.

Article Snippet: The expression plasmid pRC/CMV STAT1 Y701F was obtained from Addgene, provided by J. Darnell (Laboratory of Molecular Cell Biology, Rockefeller University, New York) and previously described (24).

Techniques: Inhibition, Expressing, Activity Assay, Transfection, Isolation, Infection, Control, Luciferase, Western Blot, Phospho-proteomics